Publication Genomics, Acloque & al.
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Publication Genomics, Acloque & al.

Understanding pig embryo development: an asset for breeding tomorrow's animals

In a study published in Genomics, scientists from the UMR Génétique Animale et Biologie Intégrative - GABI (INRAE/AgroParisTech/UPSaclay, Jouy-en-Josas) have characterized the transcriptome of 35,000 embryonic cells derived from pig embryos between 5 and 11 days after fertilization. They were able to identify new sub-populations of embryonic cells, both for the secretion of molecules required for implantation, such as those expressing interleukin 1 beta (IL1B), and a population of stem cells expressing LRP2, which will subsequently contribute to the development of the embryonic placenta.

They confirmed the existence in pigs of two types of embryonic pluripotent cells: one very early, dependent on the IL6/STAT3 pathway, and another, later, detectable from 7 days of development, which changes little over time. The dynamics of these two cell populations are associated with significant changes in the protein content of uterine fluids. Integration of the data characterizing these 35,000 cells then enabled us to identify signaling pathways in these cells capable of activating gene regulatory modules, some known to maintain the identity of pluripotent cells in vivo or in vitro, but others being completely new.

These original results will enable us to better understand the biology of the early mammalian embryo, mirroring current knowledge of human and murine embryonic cells, and to optimize derivation and culture conditions for porcine embryonic pluripotent cells. These advances will enable us to better phenotype animals in vitro, in order to meet the major challenges of the agro-ecological transition.

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See also

Adrien Dufour, Cyril Kurylo, Jan B. Stöckl, Denis Laloë, Yoann Bailly, Patrick Manceau, Frédéric Martins, Ali G. Turhan, Stéphane Ferchaud, Bertrand Pain, Thomas Fröhlich, Sylvain Foissac, Jérôme Artus, Hervé Acloque. 2024. Cell specification and functional interactions in the pig blastocyst inferred from single-cell transcriptomics and uterine fluids proteomics,
Genomics, Volume 116, Issue 2, 2024, 110780, ISSN 0888-7543, https://doi.org/10.1016/j.ygeno.2023.110780 

Modification date: 29 January 2024 | Publication date: 29 January 2024 | By: PH